CDR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58345

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Primate

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all CDR2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of human CDR2 [Swiss-Prot# Q01850]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (85%).

Localization

Cytoplasmic and Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDR2 Antibody

Western Blot: CDR2 Antibody [NB110-58345]

Western Blot: CDR2 Antibody [NB110-58345]

Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.
Immunohistochemistry: CDR2 Antibody [NB110-58345]

Immunohistochemistry: CDR2 Antibody [NB110-58345]

Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.
Flow Cytometry: CDR2 Antibody [NB110-58345]

Flow Cytometry: CDR2 Antibody [NB110-58345]

Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.
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Applications for CDR2 Antibody

Application
Recommended Usage

Flow Cytometry

reported by customer review

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at ~51 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDR2

CDR2 (cerebellar degeneration-related protein 2; encoded by gene CDR2 or PCD17) was initially discovered in a search for novel PHD1-interacting proteins, and comprehensive analysis of human cancers revealed very strong CDR2 expression in papillary subtype of renal cell carcinoma, wherein high CDR2 protein levels correlated with strongly attenuated expression of HIF target genes. Accordingly, CDR2 overexpression in cancer cell lines reduced HIF-dependent transcriptional regulation, possibly because of both regulation of PHD1 protein expression and suppression of transactivation potential of HIF-1alpha. CDR2 through its leucine zipper motif has been shown to interact with CMYC, cell cycle-related proteins and with protein kinases suggesting its important role in biological functions including signal transduction, gene transcription and cellular proliferation.

Alternate Names

CDR62, cerebellar degeneration-related protein (62kD), cerebellar degeneration-related protein 2, cerebellar degeneration-related protein 2, 62kDa, Major Yo paraneoplastic antigen, Paraneoplastic cerebellar degeneration-associated antigen, PCD17, Yo, Yo paraneoplastic antigen

Entrez Gene IDs

1039 (Human); 12585 (Mouse)

Gene Symbol

CDR2

UniProt

Q01850 (Human)

Additional CDR2 Products

Product Documents for CDR2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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