CEACAM8/CD66b Antibody (G10F5)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77808

Novus Biologicals, part of Bio-Techne
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NB100-77808
NB100-77808SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # G10F5

Concentration

1.0 mg/ml

View all CEACAM-8/CD66b Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Predicited to cross react with Chimpanzee. Use in Mouse reported in scientific literature (PMID:33122293).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CEACAM8/CD66b Antibody (G10F5)

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.
Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.
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Applications for CEACAM8/CD66b Antibody (G10F5)

Application
Recommended Usage

Flow Cytometry

1ug/million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 33122293)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NB100-77808 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95, cd66b clone g10f5, cd66b G10F5, cd66b granulocyte marker, cd66b ihc, cd66b marker, cd66b neutrophil marker, cd66b neutrophils

Entrez Gene IDs

1088 (Human)

Gene Symbol

CEACAM8

UniProt

P31997

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (G10F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM8/CD66b Antibody (G10F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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