CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80664

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # G10F5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CEACAM-8/CD66b Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Predicted to cross react with Chimpanzee.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific wi
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody. Image from the standard format of this antibody.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified customer review.
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Applications for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ug/million cells

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Gene Symbol

CEACAM8

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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