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CHCHD5 Antibody (OTI3H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03608

Novus Biologicals, part of Bio-Techne
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NBP2-03608

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H3

Concentration

0.35 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHCHD5 Antibody (OTI3H3)

Western Blot: CHCHD5 Antibody (OTI3H3) [NBP2-03608]

Western Blot: CHCHD5 Antibody (OTI3H3) [NBP2-03608]

Western Blot: CHCHD5 Antibody (3H3) [NBP2-03608] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5.
Immunocytochemistry/Immunofluorescence: CHCHD5 Antibody (3H3) [NBP2-03608] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]

Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]

Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human tonsil using anti-CHCHD5 mouse monoclonal antibody.

Applications for CHCHD5 Antibody (OTI3H3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.35 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHCHD5

Alternate Names

C2orf9, chromosome 2 open reading frame 9, coiled-coil-helix-coiled-coil-helix domain containing 5, coiled-coil-helix-coiled-coil-helix domain-containing protein 5, FLJ39671, MGC11104

Entrez Gene IDs

84269 (Human)

Gene Symbol

CHCHD5

Additional CHCHD5 Products

Product Documents for CHCHD5 Antibody (OTI3H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHCHD5 Antibody (OTI3H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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