CHCHD5 Antibody (OTI3H3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03608
![Flow Cytometry: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Flow-Cytometry-NBP2-03608-img0001.jpg "Flow Cytometry: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
![Western Blot: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Western-Blot-NBP2-03608-img0002.jpg "Western Blot: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3H3
Concentration
0.35 mg/ml
Product Summary for CHCHD5 Antibody (OTI3H3)
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CHCHD5 Antibody (OTI3H3)
Western Blot: CHCHD5 Antibody (OTI3H3) [NBP2-03608]
Western Blot: CHCHD5 Antibody (3H3) [NBP2-03608] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5.
Immunocytochemistry/Immunofluorescence: CHCHD5 Antibody (3H3) [NBP2-03608] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.
![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0008.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human tonsil using anti-CHCHD5 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0003.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0004.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0005.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0006.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
![Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]](https://resources.bio-techne.com/images/products/CHCHD5-Antibody-3H3-Immunohistochemistry-Paraffin-NBP2-03608-img0007.jpg "Immunohistochemistry-Paraffin: CHCHD5 Antibody (OTI3H3) [NBP2-03608]")
Applications for CHCHD5 Antibody (OTI3H3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.35 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CHCHD5
Alternate Names
C2orf9, chromosome 2 open reading frame 9, coiled-coil-helix-coiled-coil-helix domain containing 5, coiled-coil-helix-coiled-coil-helix domain-containing protein 5, FLJ39671, MGC11104
Entrez Gene IDs
84269 (Human)
Gene Symbol
CHCHD5
Additional CHCHD5 Products
Product Documents for CHCHD5 Antibody (OTI3H3)
Product Specific Notices for CHCHD5 Antibody (OTI3H3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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