CHCHD5 Antibody (OTI3H3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72289
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3H3
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CHCHD5 Antibody (OTI3H3) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289]
Immunocytochemistry/Immunofluorescence: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289]
Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human lymphoma tissue using anti-CHCHD5 mouse monoclonal antibody.Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289]
Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CHCHD5 antibody, and then analyzed by flow cytometry.Applications for CHCHD5 Antibody (OTI3H3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CHCHD5
Alternate Names
C2orf9, chromosome 2 open reading frame 9, coiled-coil-helix-coiled-coil-helix domain containing 5, coiled-coil-helix-coiled-coil-helix domain-containing protein 5, FLJ39671, MGC11104
Gene Symbol
CHCHD5
Additional CHCHD5 Products
Product Documents for CHCHD5 Antibody (OTI3H3) - Azide and BSA Free
Product Specific Notices for CHCHD5 Antibody (OTI3H3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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