Chromogranin A Antibody (CL0166)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52869

Novus Biologicals, part of Bio-Techne
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NBP2-52869
NBP2-52869-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0166

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Chromogranin A Antibodies »

Product Summary for Chromogranin A Antibody (CL0166)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESK

Epitope

HSGFEDELSEVLENQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Chromogranin A Antibody (CL0166)

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869] - Lane 1: Marker [kDa] Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869] - staining of SH-SY 5Y cells using the Anti-CHGA monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869] - Staining of human duodenum shows strong positivity in a subset of enteroendocrine cells.
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Applications for Chromogranin A Antibody (CL0166)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CL0166)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chromogranin A Antibody (CL0166)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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