Chromogranin A Antibody (CL0166) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44001

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0166

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Chromogranin A Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P10645, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Chromogranin A Antibody (CL0166) - Azide and BSA Free

Chromogranin A Antibody (CL0166) - Azide and BSA Free Western Blot: Chromogranin A Antibody [NBP3-44001]

Western Blot: Chromogranin A Antibody [NBP3-44001]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Chromogranin A Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]

Analysis in human parathyroid gland and liver tissues using NBP3-44001 antibody. Corresponding Chromogranin A RNA-seq data are presented for the same tissues.
Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]

Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.
View 4 more images

Applications for Chromogranin A Antibody (CL0166) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CL0166) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Chromogranin A Antibody (CL0166) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.