cIAP-2/HIAP-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-27972

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.5 mg/ml

View all cIAP-2/HIAP-1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human cIAP2 (within residues 100-200). [Swiss-Prot# Q13489]

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for cIAP-2/HIAP-1 Antibody - BSA Free

Western Blot: cIAP-2/HIAP-1 AntibodyBSA Free [NBP1-27972]

Western Blot: cIAP-2/HIAP-1 AntibodyBSA Free [NBP1-27972]

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: cIAP-2/HIAP-1 Antibody - BSA Free [NBP1-27972]

Immunocytochemistry/ Immunofluorescence: cIAP-2/HIAP-1 Antibody - BSA Free [NBP1-27972]

Immunocytochemistry/Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HIAD1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody - BSA Free [NBP1-27972]

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody - BSA Free [NBP1-27972]

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-27972] - cIAP2 staining in Oral Squamous Cell Carcinoma (OSCC). Image from verified customer review.
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Applications for cIAP-2/HIAP-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:800-1:3200

Immunohistochemistry-Paraffin

1:800-1:3200

Simple Western

1:100

Western Blot

1 ug/ml
Application Notes
This cIAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band can be seen at approx. 64 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-27972 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: cIAP-2/HIAP-1

c-IAP2 (inhibitor of apoptosis protein-2) belongs to IAPs family and besides exerting antiapoptotic effects through its interaction with multiple cytokines, it participates in inflammation, immunity and mitogenic kinase signal transduction, cell proliferation, invasion and metastasis also. With its E3 ubiquitin-protein ligase activity via interaction with several proteins such as RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, BCL10 etc., c-IAP2 can regulate NFkB signaling - positive regulation of canonical and negative regulation of non-canonical pathway. c-IAP2 participates in innate immunity via regulation of pattern recognition receptors- TLRs, NLRs and RLRs, and facilitates ubiquitination of RIPK1/CASP8 for preventing spontaneous formation of ripoptosome - a multi-protein complex which kills cancer cells in caspase-dependent/independent pathways. Knockout of c-IAP2 leads to excessive apoptosis, whereas, its overexpression has been found in several malignancies such as lymphoma and ovarian, colon, cervical cancer etc, and c-IAP2 chromosomal aberrations have been linked MALT lymphoma. c-IAP2 expression is low in normal tissues whereas in cancer its expression increases with the tumor grade and is often correlate with drug resistance, tumor recurrence, invasion and metastasis etc.

Alternate Names

BIRC3, cIAP2, HIAP-1, MIHC

Entrez Gene IDs

330 (Human)

Gene Symbol

BIRC3

UniProt

Q13489

Additional cIAP-2/HIAP-1 Products

Product Documents for cIAP-2/HIAP-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for cIAP-2/HIAP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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