Collagen VII Antibody (LH7.2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07751

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Bovine, Equine, Goat, Guinea Pig, Sheep

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LH7.2

Concentration

0.2 mg/ml

View all Collagen VII Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Collagen VII protein (Uniprot: Q02388)

Localization

Secreted. Extracellular matrix. Basement Membrane.

Specificity

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

290 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08278)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Collagen VII Antibody (LH7.2)

Immunocytochemistry/ Immunofluorescence: Collagen VII Antibody (LH7.2) [NBP3-07751]

Immunocytochemistry/ Immunofluorescence: Collagen VII Antibody (LH7.2) [NBP3-07751]

Immunocytochemistry/Immunofluorescence: Collagen VII Antibody (LH7.2) [NBP3-07751] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VIIusing Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot.
Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) [NBP3-07751]

Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) [NBP3-07751]

Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) [NBP3-07751] - Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
Flow Cytometry: Collagen VII Antibody (LH7.2) [NBP3-07751]

Flow Cytometry: Collagen VII Antibody (LH7.2) [NBP3-07751]

Flow Cytometry: Collagen VII Antibody (LH7.2) [NBP3-07751] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red)
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Applications for Collagen VII Antibody (LH7.2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Collagen VII

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Alternate Names

Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen

Gene Symbol

COL7A1

UniProt

Q02388 (Human)

Additional Collagen VII Products

Product Documents for Collagen VII Antibody (LH7.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen VII Antibody (LH7.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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