Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08278MFV450
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Bovine, Equine, Goat, Guinea Pig, Sheep
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LH7.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human Collagen VII protein (Uniprot: Q02388)
Localization
Secreted. Extracellular matrix. Basement Membrane.
Specificity
Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE]
Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE] [NBP3-08278MFV450] -
Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE] [NBP3-08278MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.Applications for Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Collagen VII
Alternate Names
Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen
Gene Symbol
COL7A1
Additional Collagen VII Products
Product Documents for Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE]
Product Specific Notices for Collagen VII Antibody (LH7.2) [mFluor Violet 450 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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