Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09025

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-09025-0.025mg
NBP3-09025-0.2mg
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Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BION-1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Common beta Chain Antibodies »

Product Specifications

Immunogen

BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.

Specificity

Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Immunocytochemistry/ Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Immunocytochemistry/Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - Human epithelial cells stained with NBP3-09025. ICC/IF image submitted by a verified customer review.
Flow Cytometry: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Flow Cytometry: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Flow Cytometry: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of BION-1 ((NBP3-09025, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/ Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Immunocytochemistry/ Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free [NBP3-09025]

Immunocytochemistry/Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of BION-1 (NBP3-09025) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09025, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

Applications for Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun 1999). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom 2002). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn 200).

Reviewed Applications

Read 1 review rated 5 using NBP3-09025 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Common beta Chain

Interleukin-3 receptor (IL-3R) is a heterodimer cytokine composed of alpha chain and beta chain. IL-3R beta subunit (CD131, beta C) is a common shared beta chain of the receptor for granulocyte- macrophage colony-stimulating factor (GM-CSF) and IL-5 (1). While the beta chain can not bind to the ligand alone, but it is essential for high affinity ligand binding (2). The cytoplasmic portion of the beta chain contains the major domains necessary for ligand-induced proliferation (3). IL-3R beta is expressed in neutrophil, eosinophil, monocyte, and hematpoietic stem cells. A point mutation leading to defective IL-3R beta expression has been associated to Human pulmonary alveolar proteinosis (PAP), a rare cause of respiratory failure (4).

Alternate Names

CD131, CSF2RB, GM-CSF R beta, IL-3 R beta, IL-5 R beta

Gene Symbol

CSF2RB

Additional Common beta Chain Products

Product Documents for Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Common beta Chain Antibody (BION-1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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