CrkL Antibody (GT466)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13625

Novus Biologicals, part of Bio-Techne
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NBP3-13625
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT466

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CrkL Antibodies »

Product Summary for CrkL Antibody (GT466)

Immunogen

Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (87%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CrkL Antibody (GT466)

Western Blot: CrkL Antibody (GT466) [NBP3-13625]

Western Blot: CrkL Antibody (GT466) [NBP3-13625]

Western Blot: CrkL Antibody (GT466) [NBP3-13625] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (NBP3-13625) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625]

Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625]

Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625]

Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625]

Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for CrkL Antibody (GT466)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CrkL

CrkL is a widely-expressed 39 kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoetic origin. Through its SH2 and SH3 domains CrkL initiates interactions with numerous downstream proteins such as CBL, Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. It is involved in processes such as adhesion, migration and immune responses. Furthermore, CrkL plays an essential role in Chronic Myelogenous Leukemia and is constitutively phosphorylated by the Bcr-Abl kinase in this disease.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Gene Symbol

CRKL

Additional CrkL Products

Product Documents for CrkL Antibody (GT466)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CrkL Antibody (GT466)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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