CrkL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-884
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KIFDPQNPDENE corresponding to C-Terminus according to NP_005198.1.
Localization
Cell membrane
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CrkL Antibody
![Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884]](https://resources.bio-techne.com/images/products/CrkL-Antibody-Immunohistochemistry-Paraffin-NB300-884-img0001.jpg "Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884]")
Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884]
Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: CrkL Antibody [NB300-884] -
Western Blot: CrkL Antibody [NB300-884] - (0.1ug/ml) staining of K562 (A) and (1ug/ml) Daudi (B) and NIH3T3 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: CrkL Antibody [NB300-884] -
Flow Cytometry: CrkL Antibody [NB300-884] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for CrkL Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry-Paraffin
2.5 - 3.8 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1-1 ug/mL
Application Notes
WB: Approx 37kDa band observed in lysates of cell lines K562, Daudi and NIH3T3 (calculated MW of 33.8kDa according to Human NP_005198.1 and Mouse NP_031790.2 ). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CrkL
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
Alternate Names
crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Gene Symbol
CRKL
UniProt
Additional CrkL Products
Product Documents for CrkL Antibody
Product Specific Notices for CrkL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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