CRTH-2/GPR44 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76755

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Guinea Pig

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CRTH-2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2. Amino Acid Squence: EQMSRLQSHSNTSIRYID

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 25917171).

Specificity

At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRTH-2/GPR44 Antibody - BSA Free

Western Blot: CRTH-2/GPR44 AntibodyBSA Free [NBP1-76755]

Western Blot: CRTH-2/GPR44 AntibodyBSA Free [NBP1-76755]

Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755]

Immunocytochemistry/ Immunofluorescence: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755]

Immunocytochemistry/Immunofluorescence: CRTH-2/GPR44 Antibody [NBP1-76755] - Human Heart tissue with CRTH2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755]

Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755]

Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-76755] - Human heart tissue with GPR44 antibody at 2.5 ug/ml.

Applications for CRTH-2/GPR44 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 25917171).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRTH-2

The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Entrez Gene IDs

11251 (Human); 14764 (Mouse); 309212 (Rat)

Gene Symbol

PTGDR2

UniProt

Q9Y5Y4

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRTH-2/GPR44 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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