CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76755CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-76755AF350

Alexa Fluor 405

NBP1-76755AF405

Alexa Fluor 488

NBP1-76755AF488

Alexa Fluor 532

NBP1-76755AF532

Alexa Fluor 594

NBP1-76755AF594

Alexa Fluor 647

NBP1-76755AF647

Alexa Fluor 700

NBP1-76755AF700

Alexa Fluor 750

NBP1-76755AF750

Biotin

NBP1-76755B

DyLight 350

NBP1-76755UV

DyLight 405

NBP1-76755V

DyLight 488

NBP1-76755G

DyLight 550

NBP1-76755R

DyLight 594

NBP1-76755DL594

DyLight 650

NBP1-76755C

DyLight 680

NBP1-76755FR

DyLight 755

NBP1-76755IR

FITC

NBP1-76755F

HRP

NBP1-76755H

Janelia Fluor 525

NBP1-76755JF525

Janelia Fluor 549

NBP1-76755JF549

Janelia Fluor 585

NBP1-76755JF585

Janelia Fluor 635

NBP1-76755JF635

Janelia Fluor 646

NBP1-76755JF646

Janelia Fluor 669

NBP1-76755JF669

mFluor Violet 450 SE

NBP1-76755MFV450

mFluor Violet 500 SE

NBP1-76755MFV500

mFluor Violet 610 SE

NBP1-76755MFV610

PerCP

NBP1-76755PCP

Forumulation

Catalog #

BSA Free

NBP1-76755

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRTH-2 Antibodies »

Product Summary for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2. Amino Acid Squence: EQMSRLQSHSNTSIRYID

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 25917171).

Specificity

At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CRTH-2

GPR44, also called CRTH2, is a Prostanoid Receptor that binds prostaglandin D2. PGD2 signals through CRTH2 to induce Th2, eosinophil, and basophil chemotaxis in a G-alpha(i) dependent manner. CRTH2 may play a role in mast cell-mediated immune responses such as those involved in allergic reactions. This receptor is upregulated in activated T helper-2 (Th2) cells, peripheral blood basophils, and eosinophils, but not in Th1 lineage cells. It may be a useful marker for Th2 and Tc2 cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated, a 3.5-kb transcript expressed primarily in the thalamus, frontal cortex, pons, and hippocampus, and a 3.4-kb transcript expressed in fetal liver, leukocytes, and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis, and thymus libraries.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Gene Symbol

PTGDR2

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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