Cyclin B1 Antibody (SPM619)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44688
![Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-SPM619-Immunocytochemistry-Immunofluorescence-NBP2-44688-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM619
Concentration
0.2 mg/ml
Product Summary for Cyclin B1 Antibody (SPM619)
Immunogen
Recombinant human full-length Cyclin B1 protein (Uniprot: P14635)
Localization
Cytoplasmic & Nuclear
Specificity
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11598)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cyclin B1 Antibody (SPM619)
Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688]
Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 MAb (SPM619)followed by Goat anti-mouse IgG-CF488 (Green).![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-SPM619-Immunohistochemistry-Paraffin-NBP2-44688-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688]")
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688]
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (SPM619)Applications for Cyclin B1 Antibody (SPM619)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cyclin B1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody (SPM619)
Product Specific Notices for Cyclin B1 Antibody (SPM619)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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