Cyclin B1 Antibody (SPM619) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48126
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) - IHC-Prediluted [NBP2-48126]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-SPM619-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48126-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) - IHC-Prediluted [NBP2-48126]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM619
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cyclin B1 Antibody (SPM619) - IHC-Prediluted
Immunogen
Recombinant human full-length Cyclin B1 protein (Uniprot: P14635)
Localization
Cytoplasmic & Nuclear
Specificity
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cyclin B1 Antibody (SPM619) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) - IHC-Prediluted [NBP2-48126]
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) - IHC-Prediluted [NBP2-48126] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (SPM619)Applications for Cyclin B1 Antibody (SPM619) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cyclin B1
Alternate Names
CCNB1
Gene Symbol
CCNB1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody (SPM619) - IHC-Prediluted
Product Specific Notices for Cyclin B1 Antibody (SPM619) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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