Cytokeratin, LMW Antibody (AE-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29411

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-1

Concentration

0.2 mg/ml

View all Cytokeratin, LMW Antibodies »

Product Specifications

Immunogen

Human epidermal keratin (Uniprot: Q7Z794)

Reactivity Notes

Turtle

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33178)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, LMW Antibody (AE-1)

Western Blot: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411]

Western Blot: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411]

Western Blot: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411] - Analysis of A431 lysate using Cytokeratin antibody at 0.25 ug/ml.
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411] - Formalin-fixed, paraffin-embedded skin stained with Cytokeratin, LMW Ab (AE1)

Applications for Cytokeratin, LMW Antibody (AE-1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, LMW

This cytokeratin antibody recognizes most of the acidic (Type I) keratins. seen by western blot as bands between 40 and 56.5 kD. In keratinized epidermis 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers. The cytokeratin AE1 antibody can be used to stain distinct cell layers in epithelial tissue and various neoplasms of epithelial origin.

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

UniProt

P04264 (Human), Q7Z794 (Human)

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (AE-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin, LMW Antibody (AE-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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