Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33178

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin, LMW Antibodies »

Product Specifications

Immunogen

Human epidermal keratin (Uniprot: Q7Z794)

Reactivity Notes

Turtle

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29411).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free

Western Blot: Cytokeratin, LMW Antibody (AE-1)Azide and BSA Free [NBP2-33178]

Western Blot: Cytokeratin, LMW Antibody (AE-1)Azide and BSA Free [NBP2-33178]

Western Blot: Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free [NBP2-33178] - Analysis of A431 lysate using Cytokeratin antibody at 0.25 ug/ml.
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free [NBP2-33178]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free [NBP2-33178]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free [NBP2-33178] - Formalin-fixed, paraffin-embedded skin stained with Cytokeratin, LMW Ab (AE1)

Applications for Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin, LMW

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin, LMW Antibody (AE-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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