Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Equine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 10D774

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all D4-GDI/RhoGDI2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for D4-GDI/RhoGDI2 Antibody (10D774)

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.
Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI.
Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.

Applications for D4-GDI/RhoGDI2 Antibody (10D774)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 25623267)

Western Blot

1-2 ug/ml
Application Notes
A 28 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.02% gelatin.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: D4-GDI/RhoGDI2

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Alternate Names

ARHGDIB Rho GDP dissociation inhibitor (GDI) beta, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2

Gene Symbol

ARHGDIB

Additional D4-GDI/RhoGDI2 Products

Product Documents for D4-GDI/RhoGDI2 Antibody (10D774)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for D4-GDI/RhoGDI2 Antibody (10D774)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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