DEK Antibody (20D2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26624
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 20D2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human DEK [UniProt P35659]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DEK Antibody (20D2)
Immunohistochemistry: DEK Antibody (20D2) [NBP3-26624] -
Immunohistochemistry: DEK Antibody (20D2) [NBP3-26624] - Image of DEK Antibody (20D2) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.67% DAB.
Immunohistochemistry: DEK Antibody (20D2) [NBP3-26624] -
Immunohistochemistry: DEK Antibody (20D2) [NBP3-26624] - Image of DEK Antibody (20D2) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.67% DAB.
Immunocytochemistry/Immunofluorescence: DEK Antibody (20D2) [NBP3-26624] -
Immunocytochemistry/Immunofluorescence: DEK Antibody (20D2) [NBP3-26624] - Staining of Hela with DEK Antibody (20D2) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 524-conjugated Goat Anti-Rabbit IgG (H+L).Applications for DEK Antibody (20D2)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: DEK
Alternate Names
D6S231EDEK oncogene (DNA binding), DEK oncogene, protein DEK
Gene Symbol
DEK
Additional DEK Products
Product Documents for DEK Antibody (20D2)
Product Specific Notices for DEK Antibody (20D2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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