DEK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98888
![Western Blot: DEK Antibody [NBP2-98888]](https://resources.bio-techne.com/images/products/DEK-Antibody-Western-Blot-NBP2-98888-img0003.jpg "Western Blot: DEK Antibody [NBP2-98888]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human DEK.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for DEK Antibody
Western Blot: DEK Antibody [NBP2-98888]
Western Blot: DEK Antibody [NBP2-98888] - Lane A: HeLa Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:50 kDa![Immunocytochemistry/ Immunofluorescence: DEK Antibody [NBP2-98888]](https://resources.bio-techne.com/images/products/DEK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98888-img0002.jpg "Immunocytochemistry/ Immunofluorescence: DEK Antibody [NBP2-98888]")
Immunocytochemistry/ Immunofluorescence: DEK Antibody [NBP2-98888]
Immunocytochemistry/Immunofluorescence: DEK Antibody [NBP2-98888] - Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DEK polyclonal antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.![Immunoprecipitation: DEK Antibody [NBP2-98888]](https://resources.bio-techne.com/images/products/DEK-Antibody-Immunoprecipitation-NBP2-98888-img0004.jpg "Immunoprecipitation: DEK Antibody [NBP2-98888]")
Immunoprecipitation: DEK Antibody [NBP2-98888]
Immunoprecipitation: DEK Antibody [NBP2-98888] - Lane A:0.5 mg K562 Whole Cell Lysate1 uL anti-DEK rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DEK rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDaApplications for DEK Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1500-1:50000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEK
Alternate Names
D6S231EDEK oncogene (DNA binding), DEK oncogene, protein DEK
Gene Symbol
DEK
Additional DEK Products
Product Documents for DEK Antibody
Product Specific Notices for DEK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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