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DIDO1 Antibody (3B1)

Novus Biologicals, part of Bio-Techne | Catalog # H00011083-M04

Novus Biologicals, part of Bio-Techne
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H00011083-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DIDO1 (AAH14489, 321 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.ILQVQDETHSETADQQEAKWRPGDADGTDCTSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILK

Reactivity Notes

This product is reactive against Human.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DIDO1 Antibody (3B1)

Western Blot: DIDO1 Antibody (3B1) [H00011083-M04]

Western Blot: DIDO1 Antibody (3B1) [H00011083-M04]

Western Blot: DIDO1 Antibody (3B1) [H00011083-M04] - Analysis of DIDO1 expression in HepG2 (Cat # L019V1).
Immunocytochemistry/ Immunofluorescence: DIDO1 Antibody (3B1) [H00011083-M04]

Immunocytochemistry/ Immunofluorescence: DIDO1 Antibody (3B1) [H00011083-M04]

Immunocytochemistry/Immunofluorescence: DIDO1 Antibody (3B1) [H00011083-M04] - Analysis of monoclonal antibody to DIDO1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: DIDO1 Antibody (3B1) [H00011083-M04] - Detection limit for recombinant GST tagged DIDO1 is 0.03 ng/ml as a capture antibody.

Applications for DIDO1 Antibody (3B1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DIDO1

Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.

Long Name

Death Inducer Obliterator 1

Alternate Names

BYE1, C20orf158, DATF1, DIO1

Gene Symbol

DIDO1

Additional DIDO1 Products

Product Documents for DIDO1 Antibody (3B1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIDO1 Antibody (3B1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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