Skip to main content

Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2010

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-2010-0.1mg

Key Product Details

Species Reactivity

Porcine

Applications

ELISA, Immunoprecipitation, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Dihydrolipoamide Dehydrogenase/DLD [Porcine Heart] (Uniprot: P09623)

Reactivity Notes

Cross reactivity against Dihydrolipoamide Dehydrogenase/DLD from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]

Applications for Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by ELISA and Western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Dihydrolipoamide Dehydrogenase/DLD

Lipoamide dehydrogenase or dihydrolipoamide dehydrogenase (DLD) is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. It is a homodimer. It belongs to the class I pyridine nucleotide disulfide oxidoreductase family. Defects in DLD are a cause of congenital infantile lactic acidosis. Moreover, defects in DLD are the cause of DLD deficiency; which results in extensive metabolic disturbances, including lactic acidemia, Krebs cycle dysfunction, and impaired branched amino acid degradation. DLD deficiency also causes neurological degeneration due to the sensitivity of the central nervous system to defects in oxidative metabolism.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Gene Symbol

DLD

UniProt

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents for Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...