Dihydrolipoamide Dehydrogenase/DLD Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77778
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778]](https://resources.bio-techne.com/images/products/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-77778-img0001.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778]")
Key Product Details
Species Reactivity
Validated:
Porcine
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Dihydrolipoamide Dehydrogenase/DLD Antibody
Immunogen
Dihydrolipoamide Dehydrogenase/DLD [Porcine Heart] (Uniprot: P09623)
Reactivity Notes
Cross reactivity against Dihydrolipoamide Dehydrogenase/DLD from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Dihydrolipoamide Dehydrogenase/DLD Antibody
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778]
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778] - Western Blot of Rabbit Dihydrolipoamide Dehydrogenase/DLD antibody. Lane 1: Dihydrolipoamide Dehydrogenase/DLD Reduced [0.05ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Dihydrolipoamide Dehydrogenase/DLD Non-Reduced [0.05ug]. Primary Antibody: Anti-Dihydrolipoamide Dehydrogenase/DLD at 1:1000 overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP at 1:40,000 for 30mins at RT. Blocking: Blocking buffer for 1hr at RT. Expected MW: 55kDa. Exp: 10 secs.Applications for Dihydrolipoamide Dehydrogenase/DLD Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Dihydrolipoamide Dehydrogenase/DLD
Alternate Names
Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3
Gene Symbol
DLD
UniProt
Additional Dihydrolipoamide Dehydrogenase/DLD Products
Product Documents for Dihydrolipoamide Dehydrogenase/DLD Antibody
Product Specific Notices for Dihydrolipoamide Dehydrogenase/DLD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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