Dkk-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-810

Novus Biologicals, part of Bio-Techne
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NB300-810
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Dkk-2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ATYSSKARLHVCQKI corresponding to C-Terminus according to NP_055236.1.

Specificity

From sequence analysis it is possible that this product will crossreact with DKK4, unfortunately this has not yet been specifically tested.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Dkk-2 Antibody

Western Blot: Dkk-2 Antibody [NB300-810]

Western Blot: Dkk-2 Antibody [NB300-810]

Western Blot: Dkk-2 Antibody [NB300-810] - NB300-810 (2 ug/ml) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Dkk-2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human heart lysate (calculated MW of 28.5 kDa band according to NP_055236).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dkk-2

Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Long Name

Dickkopf-2

Alternate Names

Dkk2

Entrez Gene IDs

27123 (Human)

Gene Symbol

DKK2

UniProt

NP_055236. (Human)

Additional Dkk-2 Products

Product Documents for Dkk-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dkk-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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