Dkk-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1000
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence RQHARLRVCQKIEKL corresponding to C-Terminus according to NP_055235.1.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Dkk-4 Antibody
Western Blot: Dkk-4 Antibody [NB100-1000] -
Western Blot: Dkk-4 Antibody [NB100-1000] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Applications for Dkk-4 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 30-35 kDa band observed in Jurkat lysate. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa band [form (i)], 30-32 kDa band [form (ii)] and 15-17 kDa band [form (iii)]. We believe will recognise 30-32 kDa band species of DKK4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Dkk-4
Additional Dkk-4 Products
Product Documents for Dkk-4 Antibody
Product Specific Notices for Dkk-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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