DNA-PKcs [p Ser2056] Antibody (JE41-92)

Western Blot: DNA-PKcs [p Ser2056] Antibody (JE41-92) [NBP3-32759]

Western Blot: DNA-PKcs [p Ser2056] Antibody (JE41-92) [NBP3-32759] - Western blot analysis of DNA-PKcs on different lysates with Rabbit anti-DNA-PKcs antibody (NBP3-32759) at 1/1,000 dilution.

Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with UV for 3 hours cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 469 kDa
Observed band size: 469 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32759) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody (JE41-92) [NBP3-32759]

Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody (JE41-92) [NBP3-32759] - Immunocytochemistry analysis of HT-29 cells treated with or without UV for 3 hours labeling DNA-PKcs with Rabbit anti-DNA-PKcs antibody (NBP3-32759) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DNA-PKcs antibody (NBP3-32759) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.