Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Monkey (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DNAM-1/CD226 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 286 to 336 of human DNAX Accessory Molecule 1 using the numbering given in entry NP_006557.2 (GeneID 10666).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAM-1/CD226 Antibody

Western Blot: DNAM-1/CD226 Antibody [NBP2-32247]

Western Blot: DNAM-1/CD226 Antibody [NBP2-32247]

Western Blot: DNAM-1/CD226 Antibody [NBP2-32247] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD226 antibody NBP2-32247 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DNAM-1/CD226 Antibody [NBP2-32247]

Immunoprecipitation: DNAM-1/CD226 Antibody [NBP2-32247]

Immunoprecipitation: DNAM-1/CD226 Antibody [NBP2-32247] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD226 antibody NBP2-32247 used for IP at 6 ug per reaction. CD226 was also immunoprecipitated by rabbit anti-CD226 antibody BL16224. For blotting immunoprecipitated CD226, NBP2-32247 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DNAM-1/CD226 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNAM-1/CD226

CD226 is a 65kD type I transmembrane glycoprotein, known as DNAM-1 (DNAX accessory molecule-1), PTA1(platelet and T cell activation antigen 1), or TLiSA1 (T lineage-specific activation antigen 1 antigen). It belongs to immunoglobulin superfamily containing 2 Ig-like domains, is expressed on majority of T lymphocytes, NK cells, monocytes, platelets, and a subset of B cells, activated endothelial cells. DNAM-1 is also expressed on CD8+ (but not CD8-) dendritic cells in mouse model. CD226 is an adhesion molecule involved in CTL and NK cell-mediated cytotoxicity. It has been identified that CD155 (poliovirus receptor, PVR) and CD112 (nectin-2, PRR-2) are CD226 ligands. DX11 antibody is able to inhibit CTL and NK cell-mediated cytotoxicity, and to block T cell cytokine production.

Long Name

DNAX Accessory Molecule 1

Alternate Names

CD226, DNAM1, PTA1, TLiSA1

Gene Symbol

CD226

UniProt

NP_006557.2 (Human)

Additional DNAM-1/CD226 Products

Product Documents for DNAM-1/CD226 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DNAM-1/CD226 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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