Dnmt2 Antibody (102B1259.2)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-587

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Flow Cytometry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 102B1259.2

Concentration

0.5 mg/ml

View all Dnmt2 Antibodies »

Product Specifications

Immunogen

This antibody was raised against synthetic peptides corresponding to mouse Dnmt2.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 17289917).

Localization

Nuclear

Specificity

In mouse Dnmt2 transfected cell lysate, a 42 kDa band is oberseved.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Dnmt2 Antibody (102B1259.2)

Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587]

Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587]

Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.
Flow Cytometry: Dnmt2 Antibody (102B1259.2) [NB200-587]

Flow Cytometry: Dnmt2 Antibody (102B1259.2) [NB200-587]

Flow Cytometry: Dnmt2 Antibody (102B1259.2) [NB200-587] - An intracellular stain was performed on Jurkat cells with Dnmt2 Antibody [102B1259.2] NB200-587 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Applications for Dnmt2 Antibody (102B1259.2)

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB200-587 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dnmt2

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Alternate Names

DMNT2, DNA (cytosine-5-)-methyltransferase 2, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, DNA methyltransferase-2, DNA MTase homolog HsaIIP, DNMT2, EC 2.1.1, EC 2.1.1.29, M.HsaIIP, MHSAIIP, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferase, tRNA aspartic acid methyltransferase 1

Entrez Gene IDs

1787 (Human); 13434 (Mouse)

Gene Symbol

TRDMT1

UniProt

Q6ICS7 (Human)

Additional Dnmt2 Products

Product Documents for Dnmt2 Antibody (102B1259.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dnmt2 Antibody (102B1259.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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