Doublecortin Antibody (OTI4A3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03081
![Western Blot: Doublecortin Antibody (OTI4A3) [NBP2-03081]](https://resources.bio-techne.com/images/products/Doublecortin-Antibody-4A3-Western-Blot-NBP2-03081-img0002.jpg "Western Blot: Doublecortin Antibody (OTI4A3) [NBP2-03081]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4A3
Concentration
0.73 mg/ml
Product Summary for Doublecortin Antibody (OTI4A3)
Immunogen
Full length human recombinant protein of human DCX (NP_835365) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Marker
Immature Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Doublecortin Antibody (OTI4A3)
Western Blot: Doublecortin Antibody (OTI4A3) [NBP2-03081]
Western Blot: Doublecortin Antibody (4A3) [NBP2-03081] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecortin.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (4A3) [NBP2-03081] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.
![Flow Cytometry: Doublecortin Antibody (OTI4A3) [NBP2-03081]](https://resources.bio-techne.com/images/products/Doublecortin-Antibody-4A3-Flow-Cytometry-NBP2-03081-img0001.jpg "Flow Cytometry: Doublecortin Antibody (OTI4A3) [NBP2-03081]")
Flow Cytometry: Doublecortin Antibody (OTI4A3) [NBP2-03081]
Flow Cytometry: Doublecortin Antibody (4A3) [NBP2-03081] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for Doublecortin Antibody (OTI4A3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.73 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Doublecortin/DCX
Long Name
Doublecortin
Alternate Names
DBCN, Doublecortex, Doublin, Lis-X, LISX
Entrez Gene IDs
1641 (Human)
Gene Symbol
DCX
Additional Doublecortin/DCX Products
Product Documents for Doublecortin Antibody (OTI4A3)
Product Specific Notices for Doublecortin Antibody (OTI4A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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