Doublecortin Antibody (OTI4A3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70558

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Doublecortin/DCX Antibodies »

Product Summary for Doublecortin Antibody (OTI4A3) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human DCX (NP_835365) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Doublecortin Antibody (OTI4A3) - Azide and BSA Free

Western Blot: Doublecortin Antibody (OTI4A3)Azide and BSA Free [NBP2-70558]

Western Blot: Doublecortin Antibody (OTI4A3)Azide and BSA Free [NBP2-70558]

Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558]

Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558]

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.
Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558]

Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558]

Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Doublecortin Antibody (OTI4A3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Doublecortin/DCX

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Gene Symbol

DCX

Additional Doublecortin/DCX Products

Product Documents for Doublecortin Antibody (OTI4A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Doublecortin Antibody (OTI4A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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