Drosophila Smad2 Antibody

Detection ofDrosophilaSmad2 by Western Blot.

Western blot shows recombinantDrosophilaSmad2 (2 ng/lane). PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Smad2 (C-terminal fragment) at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection ofDrosophilaSmad2 by Western Blot.

Western blot shows wild-type and Smad2 null mutationDrosophilalarval extracts. PVDF membrane was probed with 0.7 µg/mL of Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by Anti-Sheep IgG Secondary Antibody. A specific band was detected for Smad2 at the expected mobility of approximately 58 kDa, but not mutant extracts. This experiment was conducted under reducing conditions. dSmad2 null mutation is described in Peterson, AJ,et al.(2012) PLoS One7: e36548.Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

Smad2 inDrosophilaLarvae.

Smad2 was detected in wild-type and Smad2 null mutationDrosophilalarvae using Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) at 0.7 μg/mL. Tissue was stained using a fluorescent Anti-Sheep IgG Secondary Antibody. A single confocal image in the upper panel depicts detection of endogenous Smad2 in epidermal and central nervous system cells. The dSmad2 mutant embryo in the lower panel was stained and imaged identically and reveals background staining. dSmad2 null mutation is described in Peterson, AJ,et al.(2012) PLoS One 7: e36548.Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.