E-Cadherin Antibody (CL1170)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34475

Novus Biologicals, part of Bio-Techne
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NBP2-34475
NBP2-34475-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL1170

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGA

Epitope

NWTIQYNDPTQESII

Reactivity Notes

Rat (80%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for E-Cadherin Antibody (CL1170)

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.
Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]

Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]

Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for E-Cadherin Antibody (CL1170)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CL1170)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (CL1170)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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