E-Cadherin Antibody (CL1170) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43887

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL1170

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P12830, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for E-Cadherin Antibody (CL1170) - Azide and BSA Free

E-Cadherin Antibody (CL1170) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP3-43887]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP3-43887]

Staining of BEWO cells using the Anti-E-Cadherin monoclonal antibody) .
E-Cadherin Antibody (CL1170) - Azide and BSA Free Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP3-43887]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP3-43887]

Analysis in human duodenum and cerebral cortex tissues using NBP3-43887 antibody. Corresponding E-Cadherin RNA-seq data are presented for the same tissues.
E-Cadherin Antibody (CL1170) - Azide and BSA Free Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP3-43887]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP3-43887]

Staining of human cerebral cortex shows no positivity in neuronal cells as expected.
View 4 more images

Applications for E-Cadherin Antibody (CL1170) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CL1170) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for E-Cadherin Antibody (CL1170) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.