E-Selectin/CD62E Antibody (208)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89432

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 208

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E-Selectin/CD62E Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human E-Selectin/CD62E (Accession#: NP_000441.2; Met1-Pro556).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for E-Selectin/CD62E Antibody (208)

Immunocytochemistry/ Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432]

Immunocytochemistry/ Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432]

Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human ABHD14B monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.

Applications for E-Selectin/CD62E Antibody (208)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Selectin/CD62E

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for E-Selectin/CD62E Antibody (208)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Selectin/CD62E Antibody (208)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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