E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85473

Novus Biologicals, part of Bio-Techne
Catalog #
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NB110-85473-100ug
NB110-85473-0.025mg
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0034-9J12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all E-Selectin/CD62E Antibodies »

Product Specifications

Immunogen

Cytokine-stimulated mouse E-Selectin/CD62E

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]

Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]

Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.

Applications for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:400
Application Notes
Antigen retrieval: use 20ug/ml proteinase K in PBS for 15-25 mins

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E-Selectin/CD62E

CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

20339 (Mouse)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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