EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16865

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16865-100ul
NBP3-16865-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Y4U5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EAAT1/GLAST-1 Antibodies »

Product Summary for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EAAT1/GLAST-1/SLC1A3 (P43003). GTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Analysis of extracts of various cell lines, using EAAT1/GLAST-1/SLC1A3 Rabbit mAb (NBP3-16865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Mouse spinal cord using EAAT1/GLAST-1/SLC1A3 Rabbit mAb (NBP3-16865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865]

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Analysis of extracts of Rat brain, using EAAT1/GLAST-1/SLC1A3 Rabbit mAb (NBP3-16865) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
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Applications for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EAAT1/GLAST-1

Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2. EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.

Long Name

Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1

Alternate Names

EA6, GLAST-1, GLAST1, SLC1A3

Gene Symbol

SLC1A3

Additional EAAT1/GLAST-1 Products

Product Documents for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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