EAAT1/GLAST-1/SLC1A3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84940

Novus Biologicals, part of Bio-Techne
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NBP1-84940
NBP1-84940-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EAAT1/GLAST-1 Antibodies »

Product Summary for EAAT1/GLAST-1/SLC1A3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NGEEPKMGGRMERFQQGVRKRTLLAKKKVQNITKEDVK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT1/GLAST-1/SLC1A3 Antibody

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC1A3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human cerebral cortex, kidney, skeletal muscle and thyroid gland using Anti-PDIA3 antibody (A) NBP1-84940 shows similar protein distribution across tissues to independent antibody NBP1-84939 (B).
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940]

Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.
View 4 more images

Applications for EAAT1/GLAST-1/SLC1A3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EAAT1/GLAST-1

Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2. EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.

Long Name

Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1

Alternate Names

EA6, GLAST-1, GLAST1, SLC1A3

Gene Symbol

SLC1A3

Additional EAAT1/GLAST-1 Products

Product Documents for EAAT1/GLAST-1/SLC1A3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EAAT1/GLAST-1/SLC1A3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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