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eIF4A2 Antibody (PCRP-EIF4A2-2B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07140

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-EIF4A2-2B5

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human eIF4A2 protein (Uniprot: Q14240)

Reactivity Notes

Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08883)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for eIF4A2 Antibody (PCRP-EIF4A2-2B5)

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140]

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140]

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4A2 Mouse Monoclonal Antibody (PCRP-eIF4A2-2B5).

Applications for eIF4A2 Antibody (PCRP-EIF4A2-2B5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order Ab without BSA.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: eIF4A2

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbol

EIF4A2

UniProt

Additional eIF4A2 Products

Product Documents for eIF4A2 Antibody (PCRP-EIF4A2-2B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF4A2 Antibody (PCRP-EIF4A2-2B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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