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eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08883

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-EIF4A2-2B5

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human eIF4A2 protein (Uniprot: Q14240)

Reactivity Notes

Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07140).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free [NBP3-08883]

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free [NBP3-08883]

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-08883] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4A2 Mouse Monoclonal Antibody (PCRP-eIF4A2-2B5).

Applications for eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order antibody without BSA. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: eIF4A2

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbol

EIF4A2

Additional eIF4A2 Products

Product Documents for eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF4A2 Antibody (PCRP-EIF4A2-2B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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