EMI1 Antibody (EMI1/1176) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47786

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # EMI1/1176

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all EMI1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44344).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for EMI1 Antibody (EMI1/1176) - Azide and BSA Free

Western Blot: EMI1 Antibody (EMI1/1176)Azide and BSA Free [NBP2-47786]

Western Blot: EMI1 Antibody (EMI1/1176)Azide and BSA Free [NBP2-47786]

Western Blot: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786] - Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Antibody (EMI1/1176).
Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786] - Human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).
Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - Azide and BSA Free [NBP2-47786] - Human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).
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Applications for EMI1 Antibody (EMI1/1176) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: EMI1

Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Alternate Names

EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Gene Symbol

FBXO5

Additional EMI1 Products

Product Documents for EMI1 Antibody (EMI1/1176) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EMI1 Antibody (EMI1/1176) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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