EMI1 Antibody (EMI1/1176) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48221

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # EMI1/1176

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EMI1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for EMI1 Antibody (EMI1/1176) - IHC-Prediluted

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).
Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221]

Immunohistochemistry-Paraffin: EMI1 Antibody (EMI1/1176) - IHC-Prediluted [NBP2-48221] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

Applications for EMI1 Antibody (EMI1/1176) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: EMI1

Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Alternate Names

EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Gene Symbol

FBXO5

Additional EMI1 Products

Product Documents for EMI1 Antibody (EMI1/1176) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EMI1 Antibody (EMI1/1176) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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