Endocan/ESM-1 Antibody (6D4)
Novus Biologicals, part of Bio-Techne | Catalog # H00011082-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 6D4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ESM1 (NP_008967, 85 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPR
Specificity
ESM1 - endothelial cell-specific molecule 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Endocan/ESM-1 Antibody (6D4)
Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]
Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of ESM1 expression in transfected 293T cell line by ESM1 monoclonal antibody (M02), clone 6D4.Lane 1: ESM1 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]
Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of monoclonal antibody to ESM1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]
ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Detection limit for recombinant GST tagged ESM1 is approximately 0.03ng/ml as a capture antibody.Applications for Endocan/ESM-1 Antibody (6D4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Endocan/ESM-1
Alternate Names
ESM-1, ESM1, IGFBP-rp6
Entrez Gene IDs
11082 (Human)
Gene Symbol
ESM1
OMIM
601521 (Human)
UniProt
Additional Endocan/ESM-1 Products
Product Documents for Endocan/ESM-1 Antibody (6D4)
Product Specific Notices for Endocan/ESM-1 Antibody (6D4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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