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Endocan/ESM-1 Antibody (6D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00011082-M02

Novus Biologicals, part of Bio-Techne
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H00011082-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 6D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ESM1 (NP_008967, 85 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPR

Specificity

ESM1 - endothelial cell-specific molecule 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Endocan/ESM-1 Antibody (6D4)

Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of ESM1 expression in transfected 293T cell line by ESM1 monoclonal antibody (M02), clone 6D4.Lane 1: ESM1 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of monoclonal antibody to ESM1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02]

ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Detection limit for recombinant GST tagged ESM1 is approximately 0.03ng/ml as a capture antibody.

Applications for Endocan/ESM-1 Antibody (6D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Endocan/ESM-1

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals.

Alternate Names

ESM-1, ESM1, IGFBP-rp6

Entrez Gene IDs

11082 (Human)

Gene Symbol

ESM1

OMIM

601521 (Human)

UniProt

Additional Endocan/ESM-1 Products

Product Documents for Endocan/ESM-1 Antibody (6D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Endocan/ESM-1 Antibody (6D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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