Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80718

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MJ7/18

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Endoglin/CD105 Antibodies »

Product Summary for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

Immunogen

Inflamed mouse skin

Epitope

Endothelial cell selective TGB1 binding molecule-endoglin

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

Western Blot: Endoglin/CD105 Antibody (MJ7/18)Azide and BSA Free [NBP2-80718]

Western Blot: Endoglin/CD105 Antibody (MJ7/18)Azide and BSA Free [NBP2-80718]

Western Blot: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
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Applications for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

CD105, commonly referred to as Endoglin, ENG, HHT1, and ORW1 is a membrane single-pass type I membrane protein limited to endothelial cells in all tissues except bone marrow. It is a major glycoprotein of vascular endothelium and plays a major role in the regulation of angiogenesis. CD105 acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Mutations involving CD105 have been shown to be involved with hereditary hemorrhagic telangiectasia, as well interruptions of the TGF-beta/BMP pathways leading to some cancers. Immunohistochemistry has shown that CD105 has a very high expression in blood vessels of tumors, making it a potential target that can be used in tumor imaging (PMID: 12773481). Elevated serum levels of CD105 in pregnant women have been determined a possible risk factor for preeclampsia (PMID: 24273904).

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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