Endothelial Lipase Antibody (OTI1D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02396

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D9

Concentration

0.95 mg/ml

View all Endothelial Lipase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endothelial Lipase Antibody (OTI1D9)

Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.
Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.
Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396]

Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.
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Applications for Endothelial Lipase Antibody (OTI1D9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endothelial Lipase

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq].

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Entrez Gene IDs

9388 (Human)

Gene Symbol

LIPG

Additional Endothelial Lipase Products

Product Documents for Endothelial Lipase Antibody (OTI1D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endothelial Lipase Antibody (OTI1D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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