Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71309

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Endothelial Lipase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Endothelial Lipase Antibody (OTI1D9)Azide and BSA Free [NBP2-71309]

Western Blot: Endothelial Lipase Antibody (OTI1D9)Azide and BSA Free [NBP2-71309]

Western Blot: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-End
Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309]

Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309]

Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.
Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309]

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309]

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.
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Applications for Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endothelial Lipase

Endothelial lipase (EL) is a novel member of the lipoprotein lipase gene family. Endothelial lipase is synthesized by endothelial cells and functions at the site of synthesis. EL most likely plays a physiological role in modulating lipoprotein metabolism. Overexpressed EL has been shown to reduce HDL cholesterol levels, whereas an Endothelial lipase deficiency appears to be effective in elevating HDL cholesterol levels in animals.

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Gene Symbol

LIPG

Additional Endothelial Lipase Products

Product Documents for Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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