engrailed homeobox 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2814
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NHSTTAKEGKSDSE corresponding to C-Terminus according to NP_001418.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for engrailed homeobox 2 Antibody
![Western Blot: engrailed homeobox 2 Antibody [NB100-2814]](https://resources.bio-techne.com/images/products/engrailed-homeobox-2-Antibody-Western-Blot-NB100-2814-img0004.jpg "Western Blot: engrailed homeobox 2 Antibody [NB100-2814]")
Western Blot: engrailed homeobox 2 Antibody [NB100-2814]
Western Blot: engrailed homeobox 2 Antibody [NB100-2814] - Western blot analysis of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]](https://resources.bio-techne.com/images/products/engrailed-homeobox-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2814-img0006.jpg "Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]")
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]
Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]](https://resources.bio-techne.com/images/products/engrailed-homeobox-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2814-img0005.jpg "Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]")
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]
Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for engrailed homeobox 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:4000
Western Blot
0.1 - 1 ug/mL
Application Notes
Western blot: Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Engrailed-2
Alternate Names
EN2, Engrailed2
Gene Symbol
EN2
UniProt
Additional Engrailed-2 Products
Product Documents for engrailed homeobox 2 Antibody
Product Specific Notices for engrailed homeobox 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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