engrailed homeobox 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14947

Novus Biologicals, part of Bio-Techne
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NBP2-14947
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Engrailed-2 Antibodies »

Product Specifications

Immunogen

Full length human engrailed homeobox 2 Recombinant protein.

Reactivity Notes

Mouse (84%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for engrailed homeobox 2 Antibody

Western Blot: engrailed homeobox 2 Antibody [NBP2-14947]

Western Blot: engrailed homeobox 2 Antibody [NBP2-14947]

Western Blot: engrailed homeobox 2 Antibody [NBP2-14947] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:10000.
Immunoprecipitation: engrailed homeobox 2 Antibody [NBP2-14947]

Immunoprecipitation: engrailed homeobox 2 Antibody [NBP2-14947]

Immunoprecipitation: engrailed homeobox 2 Antibody [NBP2-14947] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 ug of EN2 antibody 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Applications for engrailed homeobox 2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Engrailed-2

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq]

Alternate Names

EN2, Engrailed2

Gene Symbol

EN2

UniProt

P19622

Additional Engrailed-2 Products

Product Documents for engrailed homeobox 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for engrailed homeobox 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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