ENPP-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38945

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-38945-25ul
NBP2-38945
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ENPP-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GLKPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENPP-1 Antibody

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-38945]

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-38945]

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-38945] - Staining of human placenta shows cytoplasmic positivity in stromal cells.
ENPP-1 Antibody

Western Blot: Rabbit Polyclonal ENPP-1 Antibody [NBP2-38945]

Western Blot: Rabbit Polyclonal ENPP-1 Antibody [NBP2-38945] - Western Blot on human valvular intersticial cell samples. Experiment used precast gel, migration at 190 V and a High weight transfer with the transblot. Then the membrane was blocked with BSA 5% for 1 hour and incubated overnight with ENPP-1 Antibody at 1/1000. The next day, after 3 washing we incubated the membrane with anti-rabbit at 1/1000 for 1h at room temperature. After 2 washing we imaged at 800nm. Image from a verified customer review.

Applications for ENPP-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

Validated for Western Blot from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-38945 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENPP-1

ENPP1 has a broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. It can hydrolyze nucleoside 5' triphosphates such as ATP, GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate. It can also hydrolyze diadenosine polyphosphates and 3',5'-cAMP to AMP. It may play a role in the regulation of pyrophosphate production, the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Alternate Names

ENPP1, Ly-41 Antigen, M6S1, NPP1, NPPS, PCA1, PDNP1

Gene Symbol

ENPP1

UniProt

P22413 (Human)

Additional ENPP-1 Products

Product Documents for ENPP-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ENPP-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.